Simultaneous enhancement of multiple functional properties using evolution-informed protein design.
Fram B, Su Y, Truebridge I, Riesselman AJ, Ingraham JB, Passera A, Napier E, Thadani NN, Lim S, Roberts K, Kaur G, Stiffler MA, Marks DS, Bahl CD, Khan AR, Sander C, Gauthier NP
Nat Commun. 2024 Jun 20;15(1):5141. doi: 10.1038/s41467-024-49119-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202332 | 98.a_pSTC0-zeo | Design 98.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202333 | 98.b_pSTC0-zeo | Design 98.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202334 | 95.a_pSTC0-zeo | Design 95.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202335 | 95.b_pSTC0-zeo | Design 95.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202336 | 90.a_pSTC0-zeo | Design 90.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202337 | 90.b_pSTC0-zeo | Design 90.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202338 | 80.a_pSTC0-zeo | Design 80.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202339 | 80.b_pSTC0-zeo | Design 80.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202340 | 70.a_pSTC0-zeo | Design 70.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202341 | 70.b_pSTC0-zeo | Design 70.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202342 | 50.a_pSTC0-zeo | Design 50.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202343 | 50.b_pSTC0-zeo | Design 50.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202347 | opt.a_pSTC0-zeo | Design opt.a (greedy optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202348 | opt.b_pSTC0-zeo | Design opt.b (parallel tempering optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202349 | rw-consensus_pSTC0-zeo | Control rw-consensus (reweighted consensus) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202350 | neg.ctrl_pSTC0-zeo | Control neg. ctrl (negative control S70A) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |
| 202351 | WTTEM1_pSTC0-zeo | Control WT TEM-1 (wild type TEM-1 beta lactamase) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette |