Addgene: Simultaneous enhancement of multiple functional properties using evolution-informed protein design.

Simultaneous enhancement of multiple functional properties using evolution-informed protein design.

Fram B, Su Y, Truebridge I, Riesselman AJ, Ingraham JB, Passera A, Napier E, Thadani NN, Lim S, Roberts K, Kaur G, Stiffler MA, Marks DS, Bahl CD, Khan AR, Sander C, Gauthier NP
Nat Commun. 2024 Jun 20;15(1):5141. doi: 10.1038/s41467-024-49119-x. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
202351	WTTEM1_pSTC0-zeo	Control WT TEM-1 (wild type TEM-1 beta lactamase) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette
202350	neg.ctrl_pSTC0-zeo	Control neg. ctrl (negative control S70A) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette
202349	rw-consensus_pSTC0-zeo	Control rw-consensus (reweighted consensus) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette
202348	opt.b_pSTC0-zeo	Design opt.b (parallel tempering optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette
202347	opt.a_pSTC0-zeo	Design opt.a (greedy optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette
202343	50.b_pSTC0-zeo	Design 50.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette
202342	50.a_pSTC0-zeo	Design 50.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette
202341	70.b_pSTC0-zeo	Design 70.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette
202340	70.a_pSTC0-zeo	Design 70.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette
202339	80.b_pSTC0-zeo	Design 80.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Antibodies from Article