Redesign of an Escherichia coli Nissle treatment for phenylketonuria using insulated genomic landing pads and genetic circuits to reduce burden.
Triassi AJ, Fields BD, Monahan CE, Means JM, Park Y, Doosthosseini H, Padmakumar JP, Isabella VM, Voigt CA
Cell Syst. 2023 Jun 21;14(6):512-524.e12. doi: 10.1016/j.cels.2023.05.004.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202287 | plYJP022-int7 | Plasmid containing integrase for LPattB7 site |
| 202289 | pAJT248 | RPU integration plasmid (LPattB2) |
| 202291 | pAJT430 | RPU integration plasmid (LPattB7) |
| 202292 | pAJT475 | RPU plasmid w/ high RBS (p15a backbone) |
| 202293 | pAJT194 | Type IIS cloning plasmid for LPattB5 integrations w/ OriT |
| 202294 | pAJT195 | Type IIS cloning plasmid for LPattB7 integrations w/ OriT |
| 202297 | pAJT237-Ptac | PTac-YFP plasmid for LPattB2 integration |
| 202298 | pAJT238-Pbad | PBad-YFP plasmid for LPattB2 integration |
| 202299 | plYJP067-Ptet | PTet-YFP plasmid for LPattB2 integration |
| 202300 | plYJP067-Pttg | PTtg-YFP plasmid for LPattB2 integration |
| 202301 | pAJT243-Pcin | PCin-YFP plasmid for LPattB2 integration |
| 202302 | plYJP067-PcymRC_v2 | PCymRC_v2-YFP plasmid for LPattB2 integration |
| 202304 | pAJT249-psrA | psrA repressor plasmid for LPattB2 integration |
| 202306 | pAJT251-bm3R1 | bm3R1 repressor plasmid for LPattB2 integration |
| 202307 | pAJT252-amtR | amtR repressor plasmid for LPattB2 integration |
| 202308 | pAJT254-litR | litR repressor plasmid for LPattB2 integration |
| 202309 | pAJT255-ameRS | ameRS repressor plasmid for LPattB2 integration |