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A genetically encoded photoproximity labeling approach for mapping protein territories.

Hananya N, Ye X, Koren S, Muir TW
Proc Natl Acad Sci U S A. 2023 Apr 18;120(16):e2219339120. doi: 10.1073/pnas.2219339120. Epub 2023 Apr 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
202204pCMV-FLAG-LOV*-H2BExpresses the photoproximity labeling catalyst LOV* fused to H2B in mammalian cells
202205pCMV-PDK1-FLAG-LOV*Expresses the photoproximity labeling catalyst LOV* fused to PDK1 in mammalian cells
202206pCMV-FLAG-LOV*-MVPExpresses the photoproximity labeling catalyst LOV* fused to MVP in mammalian cells
202207pCMV-MTS-FLAG-LOV*Expresses the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells
202208pLenti-EF1a-MTS-FLAG-LOV*Plasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells

Antibodies from Article