De Novo Synthesis of a Conjugative System from Human Gut Metagenomic Data for Targeted Delivery of Cas9 Antimicrobials.
Hamilton TA, Joris BR, Shrestha A, Browne TS, Rodrigue S, Karas BJ, Gloor GB, Edgell DR
ACS Synth Biol. 2023 Dec 15;12(12):3578-3590. doi: 10.1021/acssynbio.3c00319. Epub 2023 Dec 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202033 | pCitro-15a | p15a-based plasmid that encodes a conjugative system synthesized from metagenomic data for conjugation to Citrobacter |
| 202088 | pENTR-TC9 | entry vector for gateway recombination of TevCas9 and sgRNA into a destination cassette in pCitro-dest |