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De Novo Synthesis of a Conjugative System from Human Gut Metagenomic Data for Targeted Delivery of Cas9 Antimicrobials.

Hamilton TA, Joris BR, Shrestha A, Browne TS, Rodrigue S, Karas BJ, Gloor GB, Edgell DR
ACS Synth Biol. 2023 Dec 15;12(12):3578-3590. doi: 10.1021/acssynbio.3c00319. Epub 2023 Dec 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
202033pCitro-15ap15a-based plasmid that encodes a conjugative system synthesized from metagenomic data for conjugation to Citrobacter
202088pENTR-TC9entry vector for gateway recombination of TevCas9 and sgRNA into a destination cassette in pCitro-dest

Antibodies from Article