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Assessment of Enterovirus Antibodies during Early Childhood Using a Multiplex Immunoassay.

Jouppila NVV, Lehtonen J, Seppala E, Puustinen L, Oikarinen S, Laitinen OH, Knip M, Hyoty H, Hytonen VP
Microbiol Spectr. 2023 Jun 15;11(3):e0535222. doi: 10.1128/spectrum.05352-22. Epub 2023 May 25. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
202068pGEX-2T_CVA4_VP1Bacterial expression of VP1 protein from coxsackievirus A4 (CAV4). Contains N-terminal GST-tag and C-terminal His6-tag.
202069pGEX-2T_CVB1_VP1Bacterial expression of VP1 protein from coxsackievirus B1 (CVB1). Contains N-terminal GST-tag and C-terminal His6-tag.
202070pGEX-2T_E30_VP1Bacterial expression of VP1 protein from echovirus 30 (E30). Contains N-terminal GST-tag and C-terminal His6-tag.
202071pGEX-2T_PV1_VP1Bacterial expression of VP1 protein from poliovirus 1 (PV1). Contains N-terminal GST-tag and C-terminal His6-tag.
202072pGEX-2T_EV-D68_VP1Bacterial expression of VP1 protein from enterovirus D68 (EV-D68). Contains N-terminal GST-tag and C-terminal His6-tag.
202073pGEX-2T_RV-A89_VP1Bacterial expression of VP1 protein from rhinovirus A89 (RV-A89). Contains N-terminal GST-tag and C-terminal His6-tag.
202074pGEX-2T_RV-B14_VP1Bacterial expression of VP1 protein from rhinovirus B14 (RV-B14). Contains N-terminal GST-tag and C-terminal His6-tag.
202076pBVboostFGII WPRE 2A-CVB3Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.
202077pBVboostFGII WPRE 3C-CVB3Bacterial expression of 3C protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

Antibodies from Article