Dynamic mapping of proteome trafficking within and between living cells by TransitID.
Qin W, Cheah JS, Xu C, Messing J, Freibaum BD, Boeynaems S, Taylor JP, Udeshi ND, Carr SA, Ting AY
Cell. 2023 Jun 23:S0092-8674(23)00596-2. doi: 10.1016/j.cell.2023.05.044.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202008 | Flag-TurboID-NES_pLX304 | expresses TurboID in the mammalian cytosol, lentiviral vector |
| 202013 | APEX2-V5-G3BP1_pTRE-G418 | expresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector |
| 202015 | V5-LOV-Turbo1-G3BP1_pTRE-G418 | expresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector |
| 202017 | V5-LOV-Turbo1-NIK3x_pTRE-G418 | expresses LOV-Turbo1-NIK3x in the mammalian nucleolus under dox inducible promoter, lentiviral vector |
| 202018 | V5-GFP-APEX2-NES_pLX208 | expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector |
| 202019 | Flag-GFP-TurboID-NES_pLX304 | expresses GFP-TurboID in the mammalian cytosol, lentiviral vector |
| 202020 | V5-GFP-TurboID-KDEL_pLX208 | expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector |
| 202021 | Flag-GFP-HRP-TM_pLX304 | expresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vector |