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Efficient gene activation in plants by the MoonTag programmable transcriptional activator.
Casas-Mollano JA, Zinselmeier M, Sychla A, Smanski MJ
Nucleic Acids Research, Volume 51, Issue 13, 21 July 2023, Pages 7083–7093 PubMed Article

Plasmids from Article

ID Plasmid Purpose
202005pMC-J6-dCas9-10XGP41-J9MoClo level 0 dCas9-10XGP41 coding sequence module
202006pMC-J6-dCas9-24XGP41-J9MoClo level 0 dCas9-24XGP41 coding sequence module
202007pMC-J6-NbGP41-sfGFP-VP64-GB1-J9MoClo level 0 NbGP41-sfGFP-VP64-GB1 coding sequence module
202009pMOD_A-CmYLCV-dCas9-24XGP41Moclo level 1 - Module A, Promoter: CmYLCV, Gene: dCas9-24XGP41, Terminator: HSP
202010pMOD_D-CmYLCV-NbGP41P-sfGFP-VP64-GB1Moclo level 1 - Module D, Promoter: CmYLCV, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: ZmUBI
202011pMOD_A-AtUBI10-dCas9-24XGP41Moclo level 1 - Module A, Promoter: AtUBI10, Gene: dCas9-24XGP41, Terminator: HSP
202012pMOD_D-AtUBI10-NbGP41-sfGFP-VP64-GB1Moclo level 1 - Module D, Promoter: AtUBI10, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: pea rbcsE9
202014pMT1-24X-NOG(CmYLCV)T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.
202016pMT1-24X-NOG(AtUBI10)T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

Antibodies from Article