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Alejandro Chavez Lab: Viral protease library for small molecule screening

Unpublished

Plasmids from Article

ID Plasmid Purpose
201792pAG416GAL-EYFPExpresses EYFP from a GAL promoter with a URA3 marker
201935pAG416GAL-1x-HIV-2_PRExpresses HIV-2 protease from a GAL-1x promoter with a URA3 marker
201936pAG416GAL-2x-uA-BWCoV-SW1_3CLExpresses Beluga Whale-CoV SW1 3CL protease from a GAL-2x promoter with a URA3 marker
201937pAG416GAL-3x-uC-HCoV-229E_3CLExpresses HCoV-229E 3CL protease from a GAL-3x promoter with a URA3 marker
201938pAG416GAL-3x-uC-HCoV-NL63_3CLExpresses HCoV-NL63 3CL protease from a GAL-3x promoter with a URA3 marker
201939pAG416GAL-Bat-SL-CoVZC45_PLPExpresses Bat-SL-CoVZC45 papain-like protease from a GAL promoter with a URA3 marker
201940pAG416GAL-DENV_NS3-GS-NS2BExpresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker
201941pAG416GAL-EMCV_3CExpresses EMCV 3C protease from a GAL promoter with a URA3 marker
201942pAG416GAL-SARS-CoV_PLPExpresses SARS-CoV papain-like protease from a GAL promoter with a URA3 marker
201943pAG416GAL10-Bat-CoV-HKU4_3CLExpresses Bat-CoV HKU4 3CL protease from a GAL10 promoter with a URA3 marker
201944pAG416GAL10-Bat-SL-CoVZC45_3CLExpresses Bat-SL-CoVZC45 3CL protease from a GAL10 promoter with a URA3 marker
201945pAG416GAL10-CCHFV_OTUExpresses CCHFV OTU domain of L protein from a GAL10 promoter with a URA3 marker
203462pAG416GAL10-c-SrcExpresses human c-Src kinase from a GAL10 promoter with a URA3 marker
203463pAG416GAL10-DUGV_OTUExpresses DUGV OTU domain of L protein from a GAL10 promoter with a URA3 marker
203465pAG416GAL10-HPeV_3CExpresses HPeV 3C protease from a GAL10 promoter with a URA3 marker
203466pAG416GAL10-HRV-B14_3CExpresses HRV-B14 3C protease from a GAL10 promoter with a URA3 marker
203467pAG416GAL10-HTLV_PRExpresses HTLV protease from a GAL10 promoter with a URA3 marker
203468pAG416GAL10-LGTV_NS2B-GS-NS3Expresses LGTV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker
203470pAG416GAL10-NWV_3CLExpresses NWV 3CL protease from a GAL10 promoter with a URA3 marker
203471pAG416GAL10-POWV_NS2B-GS-NS3Expresses POWV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker
203472pAG416GAL10-PV_3CExpresses PV 3C protease from a GAL10 promoter with a URA3 marker
203473pAG416GAL10-SHV_3CLExpresses SHV 3CL protease from a GAL10 promoter with a URA3 marker
203474pAG416GAL10-SV-A_3CExpresses SV-A 3C protease from a GAL10 promoter with a URA3 marker
203475pAG416GAL10-WNV_NS2B-GS-NS3Expresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker
203476pAG416GAL10-YES1-6stopExpresses human YES1 kinase from a GAL10 promoter with a URA3 marker
203477pAG416GAL10-ZIKV_NS2B-GS-NS3Expresses ZIKV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker
203478pAG416GALL-Bat-CoV-HKU9_3CLExpresses Bat-CoV HKU9 3CL protease from a GALL promoter with a URA3 marker
203481pAG416GALL-HAV_3CExpresses HAV 3C protease from a GALL promoter with a URA3 marker
203493pAG416GALL-HIV-1_PRExpresses HIV-1 protease from a GALL promoter with a URA3 marker
203495pAG416GALL-SARS-CoV_3CLExpresses SARS-CoV 3CL protease from a GALL promoter with a URA3 marker
203496pAG416GALL-SARS-CoV-2_3CLExpresses SARS-CoV-2 3CL protease from a GALL promoter with a URA3 marker
203499pAG426GAL-CV-B3_3CExpresses CV-B3 3C protease from a GAL promoter with a URA3 marker
203501pAG426GAL-FESExpresses human FES kinase from a GAL promoter with a URA3 marker
203502pAG426GAL-HCoV-NL63_PLP2Expresses HCoV-NL63 PLP2 from a GAL promoter with a URA3 marker
203503pAG426GAL-HCoV-OC43_3CLExpresses HCoV-OC43 3CL protease from a GAL promoter with a URA3 marker
203504pAG426GAL-HCV_NS3-GS-NS4AExpresses HCV NS3-NS4A protease (with GS linker) from a GAL promoter with a URA3 marker
203505pAG426GAL-HHV-3_PRExpresses protease domain of HHV-3 (VZV) from a GAL promoter with a URA3 marker
203506pAG426GAL-KUNV_NS2B-GS-NS3Expresses KUNV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker
203507pAG426GAL-LV_3CLExpresses LV 3CL protease from a GAL promoter with a URA3 marker
203508pAG426GAL-MERS-CoV_PLPExpresses MERS-CoV papain-like protease from a GAL promoter with a URA3 marker
203509pAG426GAL-SARS-CoV-2_PLPExpresses SARS-CoV-2 papain-like protease from a GAL promoter with a URA3 marker
203510pAG426GAL-SLEV_NS2B-GS-NS3Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker
203511pAG416GALL-ccdB-6stopGateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 marker
203512pAG416GAL10-ccdB-6stopGateway-compatible destination vector for yeast expression with GAL10 promoter/6stop mutation/URA3 marker
203514pAG416GAL-3x-uC-ccdB-6stopGateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker
203517pAG416GAL-2x-uA-ccdB-6stopGateway-compatible destination vector for yeast expression with GAL1 promoter (2x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker
203519pAG416GAL-1x-ccdB-6stopGateway-compatible destination vector for yeast expression with GAL1 promoter (1x GAL4 binding sites)/6stop mutation/URA3 marker

Antibodies from Article