A robust dual gene ON-OFF toggle directed by two independent promoter-degron pairs.
Yeung TK, Kim S, Ma HT, Poon RYC
J Cell Sci. 2023 Apr 15;136(8):jcs260754. doi: 10.1242/jcs.260754. Epub 2023 Apr 19.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201556 | pDBEcR/Bla | Plasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance. |
| 201557 | EGFP in pDBEcR-pIND(SP1)/Bla | Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance. |
| 201558 | EGFP in pDBEcR-pIND(SP1)-W/Bla | Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance. |
| 201559 | EGFP in pDBEcR-pIND(SP1)-DD/Bla | Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD and DBEcR (3xmyc-tagged); blasticidin resistance. |
| 201560 | EGFP in pDBEcR-pIND(SP1)-DD-W/Bla | Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance. |