Domain-inlaid Nme2Cas9 adenine base editors with improved activity and targeting scope.
Bamidele N, Zhang H, Dong X, Cheng H, Gaston N, Feinzig H, Cao H, Kelly K, Watts JK, Xie J, Gao G, Sontheimer EJ
Nat Commun. 2024 Feb 17;15(1):1458. doi: 10.1038/s41467-024-45763-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201503 | pEJS2156_Nme2-ABE8e-nt | Mammalian expression of N-terminally fused Nme2-ABE8e-nt |
| 201510 | pEJS2158_Nme2-ABE8e-i1 | Mammalian expression of domain inlaid Nme2-ABE8e-i1 |
| 201511 | pEJS2159_Nme2-ABE8e-i2 | Mammalian expression of domain inlaid Nme2-ABE8e-i2 |
| 201513 | pEJS2160_Nme2-ABE8e-i3 | Mammalian expression of domain inlaid Nme2-ABE8e-i3 |
| 201514 | pEJS2161_Nme2-ABE8e-i4 | Mammalian expression of domain inlaid Nme2-ABE8e-i4 |
| 201515 | pEJS2162_Nme2-ABE8e-i5 | Mammalian expression of domain inlaid Nme2-ABE8e-i5 |
| 201516 | pEJS2163_Nme2-ABE8e-i6 | Mammalian expression of domain inlaid Nme2-ABE8e-i6 |
| 201517 | pEJS2164_Nme2-ABE8e-i7 | Mammalian expression of domain inlaid Nme2-ABE8e-i7 |
| 201518 | pEJS2165_Nme2-ABE8e-i8 | Mammalian expression of domain inlaid Nme2-ABE8e-i8 |
| 201519 | pEJS2437_Nme2.Smu-ABE8e-nt | Mammalian expression of N-terminally fused Nme2.Smu-ABE8e-nt |
| 201520 | pEJS2422_Nme2.Smu-ABE8e-i1 | Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i1 |
| 201521 | pEJS2423_Nme2.Smu-ABE8e-i7 | Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i7 |
| 201522 | pEJS2424_Nme2.Smu-ABE8e-i8 | Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i8 |
| 201523 | pEJS2217_Nme2-rAPOBEC-nt | Mammalian expression of N-terminally fused Nme2-rAPOBEC-nt |
| 201524 | pEJS2214_Nme2-rAPOBEC-i1 | Mammalian expression of domain inlaid Nme2-rAPOBEC-i1 |
| 201525 | pEJS2215_Nme2-rAPOBEC-i7 | Mammalian expression of domain inlaid Nme2-rAPOBEC-i7 |
| 201526 | pEJS2216_Nme2-rAPOBEC-i8 | Mammalian expression of domain inlaid Nme2-rAPOBEC-i8 |
| 201527 | pEJS2191_Nme2-evoFERNY-nt | Mammalian expression of N-terminally fused Nme2-evoFERNY-nt |
| 201528 | pEJS2195_Nme2-evoFERNY-i1 | Mammalian expression of domain inlaid Nme2-evoFERNY-i1 |
| 201529 | pEJS2196_Nme2-evoFERNY-i7 | Mammalian expression of domain inlaid Nme2-evoFERNY-i7 |
| 201530 | pEJS2197_Nme2-evoFERNY-i8 | Mammalian expression of domain inlaid Nme2-evoFERNY-i8 |
| 201683 | pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI | All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers. |
| 201684 | pEJS2602_AAV-Nme2-ABE8e-i1(V106W)-U6-2xSapI | All-in-one AAV plasmid expressing Nme2-ABE8e-i1(V106W) and U6 sgRNA cassette. SapI enables cloning new spacers. |
| 201685 | pEJS2603_AAV-Nme2-ABE8e-i1-U6-Rosa26 | All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 driven sgRNA targeting the mouse Rosa26 gene |
| 201686 | pEJS2604_AAV-Nme2-ABE8e-i1(V106W)-U6-Rosa26 | All-in-one AAV plasmid expressing Nme2-ABE8e-i1 (V106W) and U6 driven sgRNA targeting the mouse Rosa26 gene |