A split GAL4 RUBY assay for visual in planta detection of protein-protein interactions.
Chen J, Luo M, Hands P, Rolland V, Zhang J, Li Z, Outram M, Dodds P, Ayliffe M
Plant J. 2023 Jun;114(6):1209-1226. doi: 10.1111/tpj.16234. Epub 2023 Apr 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201230 | GAL4BD-VP16/pDON207 | GAL4-VP16 transcription factor |
| 201231 | 35S-GWY-GAL4BD/pAM | Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD |
| 201232 | 35S-GWY-VP16/pAM | Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain |
| 201233 | 35S-GAL4BD-GWY/pAM | Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD |
| 201234 | 35S-VP16-GWY/pAM | Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain |