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Disentangling autoproteolytic cleavage from tethered agonist-dependent activation of the adhesion receptor ADGRL3.

Perry-Hauser NA, VanDyck MW, Lee KH, Shi L, Javitch JA
J Biol Chem. 2022 Dec;298(12):102594. doi: 10.1016/j.jbc.2022.102594. Epub 2022 Oct 14. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
201217pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTFpCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF
201218pCDNA3.1-ADGRL3-FlagpCDNA3.1 with kozak (GCC), ADGRL3 and C-terminal Flag
201219ADGRL3-CTF-FlagpCDNA3.1 with kozak (GCC), ADGRL3 C-terminal fragment and C-terminal Flag

Antibodies from Article