Moshe Szyf Lab: CRISPRi plasmids for reduced leakiness of doxycycline-inducible tight TRE promoter
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201151 | pLenti-dSaCas9-KRAB-gRNA-TRE-blast | Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene. |
| 201152 | pLenti-dCas9-KRAB-gRNA-TRE-blast | Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene. |