An enzyme-based biosensor for monitoring and engineering protein stability in vivo.
Ren C, Wen X, Mencius J, Quan S
Proc Natl Acad Sci U S A. 2021 Mar 30;118(13):e2101618118. doi: 10.1073/pnas.2101618118.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201080 | pTrc-CysGA-Linker only | GS linker inserted into CysGA at G364 |
| 201081 | pTrc-CysGA-Im7 WT | Im7 WT inserted into CysGA at G364 via GS linker |
| 201082 | pTrc-CysGA-Im7 F84A | Im7 F84A variant inserted into CysGA at G364 via GS linker |