Prolonging genetic circuit stability through adaptive evolution of overlapping genes.
Chlebek JL, Leonard SP, Kang-Yun C, Yung MC, Ricci DP, Jiao Y, Park DM
Nucleic Acids Res. 2023 Jun 1:gkad484. doi: 10.1093/nar/gkad484.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201531 | pJUMP24_T24_pRham-ilvA-relE | Expressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle) |
| 201532 | pJUMP24_T24_pRham-ilvA-relE_RBS3 | Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle) |
| 201533 | pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2 | Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle) |
| 201534 | pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3 | Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle) |
| 201535 | pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4 | Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle) |