Discovery and Validation of Context-Dependent Synthetic Mammalian Promoters.
Zahm AM, Owens WS, Himes SR, Rondem KE, Fallon BS, Gormick AN, Bloom JS, Kosuri S, Chan H, English JG
bioRxiv. 2023 May 11:2023.05.11.539703. doi: 10.1101/2023.05.11.539703. Preprint.
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These individual pooled libraries are also available as a kit: TRE-MPRA full library kit
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201012 | TRE-MPRA_minCMV Library | Massively parallel reporter assay (MPRA) library with minCMV promoter |
| 201013 | TRE-MPRA_minPromega Library | Massively parallel reporter assay (MPRA) library with minPromega promoter |
| 201014 | TRE-MPRA_minTK Library | Massively parallel reporter assay (MPRA) library with minTK promoter |
| 211516 | pGL4.R_TRE_minTK | Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression |
| 211517 | pGL4.R_TRE_minPro | Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression |
| 211518 | pGL4.R_TRE_minCMV | Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression |