Directed Evolution of Acoustic Reporter Genes Using High-Throughput Acoustic Screening
Hurt RC, Jin Z, Soufi M, Wong KK, Sawyer DP, Shen HK, Dutka P, Deshpande R, Zhang R, Mittelstein DR, Shapiro MG
bioRxiv 2024.03.30.587094
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202023 | pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015 | Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coli |
| 202024 | pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015 | Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coli |
| 202025 | pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015 | Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli |