Standardizing cassette-based deep mutagenesis by Golden Gate assembly.

Daffern N, Francino-Urdaniz IM, Baumer ZT, Whitehead TA
Biotechnol Bioeng. 2024 Jan;121(1):281-290. doi: 10.1002/bit.28564. Epub 2023 Sep 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
200949	pND003	Yeast surface display destination vector for Golden Gate cloning
200950	pND004	MBP-tagged E. coli protein expression destination vector for Golden Gate cloning
200951	pND005	Yeast two-hybrid destination vector for Golden Gate cloning