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The B-box1 domain of PML mediates SUMO E2-E3 complex formation through an atypical interaction with UBC9.

Bregnard T, Ahmed A, Semenova IV, Weller SK, Bezsonova I
Biophys Chem. 2022 Aug;287:106827. doi: 10.1016/j.bpc.2022.106827. Epub 2022 May 18. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
185636pGEX-PML RB1expression of GST-tagged PML fragment 49-168 (RING + B-box1) in bacterial cells
185637pGEX-SUMO1expression of GST-tagged SUMO1 in bacterial cells

Antibodies from Article