CasPlay provides a gRNA-barcoded CRISPR-based display platform for antibody repertoire profiling.
Barber KW, Shrock E, Elledge SJ
Cell Rep Methods. 2022 Oct 17;2(10):100318. doi: 10.1016/j.crmeth.2022.100318. eCollection 2022 Oct 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186420 | dCas9 fused to nanobody (anti-beta catenin) | Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag |
| 186421 | dCas9 fused to scFv (anti-spike) | Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2 |
| 186422 | GST-beta catenin tag | Encodes GST fused to beta catenin tag recognized by anti-beta catenin nanobody |