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Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly.

Ratto E, Chowdhury SR, Siefert NS, Schneider M, Wittmann M, Helm D, Palm W
Nat Commun. 2022 Aug 17;13(1):4848. doi: 10.1038/s41467-022-32515-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
228864pLV_3XHA-ATP6V1B2Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA
228865pLV_3XHA-ATP6V1B2-mNeonGreenStable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen
228866pLV_3xFLAG-ATP6V0A3Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG
228867pLV_3xFLAG-ATP6V0A3-mScarletStable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

Antibodies from Article