Applications of an inactive Dispersin B probe to monitor biofilm polysaccharide production.
Eddenden A, Nitz M
Methods Enzymol. 2022;665:209-231. doi: 10.1016/bs.mie.2021.11.006. Epub 2021 Dec 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176573 | pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis | Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG. |
| 176575 | pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis | Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG. |
| 176576 | pET29b-mVenus-dspB(E184Q)-6xHis | Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG. |
| 176577 | pET29b-mVenus-dspB(E184Q W330Y)-6xHis | Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG. |