ALIBY: ALFA Nanobody-Based Toolkit for Imaging and Biochemistry in Yeast.
Akhuli D, Dhar A, Viji AS, Bhojappa B, Palani S
mSphere. 2022 Oct 26;7(5):e0033322. doi: 10.1128/msphere.00333-22. Epub 2022 Oct 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201764 | pABY-T1 | For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast |
| 201765 | pABY-T2 | For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast |
| 201766 | pABY-T3 | For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast |
| 201767 | pABY-T4 | For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast |
| 201768 | pABY-c15 | Expresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeast |
| 201769 | pABY-c25 | Expresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeast |
| 201771 | pABY-c45 | Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast |
| 201774 | pABY-c46 | Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast |