A specific role for importin-5 and NASP in the import and nuclear hand-off of monomeric H3.
Pardal AJ, Bowman AJ
Elife. 2022 Sep 6;11:e81755. doi: 10.7554/eLife.81755.
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PubMed
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217955 | pCL1313 | Expression of human H3.1 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter. |
| 217956 | pCL1314 | Expression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter. |
| 217957 | pCL1315 | Expression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter. |
| 217958 | pCL1316 | Expression of human H4 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter. |
| 217959 | pCL1317 | Expression of human H4 carrying a triple glycine insertion to disrupt folding with H3. Expression in mammalian cells driven by the CMV promoter. |
| 217960 | pCL1318 | Expression of human H4 carrying three point mutations to disrupt binding with H3. Expression in mammalian cells driven by the CMV promoter. |
| 217961 | pCL1177 | Dual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin. |
| 217964 | pCL1161 | For RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher). |
| 217965 | pCL1324 | Bacterial expression of Importin-5 tagged on its N-terminus with GST. Cleavable with 3C protease. |
| 217966 | pCL1333 | Bacterial expression of RAN with an N-terminal 6His tag and a TEV cleavage site. |