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PI(4,5)P2 diffuses freely in the plasma membrane even within high-density effector protein complexes.

Pacheco J, Cassidy AC, Zewe JP, Wills RC, Hammond GRV
J Cell Biol. 2023 Feb 6;222(2):e202204099. doi: 10.1083/jcb.202204099. Epub 2022 Nov 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
202677PAmCherry1-N1-TubbycPhotoactivatable lipid biosensor
202678PAmCherry-N1-FRB-TubbycPhotoactivatable and rapamycin recruitable lipid biosensor
202679PAmCherry1-N1-PHPLCd1Photoactivatable lipid biosensor
202680PAmCherry1-C1-P4CPhotoactivatable lipid biosensor
202681PAmCherry1-C1-P4Mx2Photoactivatable lipid biosensor
202682PAmCherry1-C1-LACT-C2Photoactivatable lipid biosensor
202683PAmCherry1-N1-Lyn11Photoactivatable lipid biosensor
202684EGFP-N-Ezrinfluorescent protein tagged ezrin
202685EGFP-C3-beta-spectrinfluorescent protein tagged beta-spectrin
202686EGFP-C1-FKBP-CYB5tailrapamycin-induced recruitment of proteins to ER

Antibodies from Article