Modularity and diversity of target selectors in Tn7 transposons.
Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200840 | pMSIP116: pHelper(CyCAST)_PSP1 | Inducible expression of CyCAST proteins with crRNA targeting CyPSP1. |
| 200841 | pMSIP122: pHelper(CyCAST)_Shuffled-spacer | Inducible expression of CyCAST proteins with control crRNA for CyPSP1. |
| 200842 | pMSIP267: pHelper(CyCAST)_PSP1_ΔTniQ | Inducible expression of CyCAST proteins (except TniQ) with crRNA targeting CyPSP1. |
| 200844 | pMSIP269: pHelper(CyCAST)_PSP1_ΔTnsD | Inducible expression of CyCAST proteins (except TnsD) with crRNA targeting CyPSP1. |
| 200845 | pMSIP270: pHelper(CyCAST)_Shuffled-spacer_ΔTnsD | Inducible expression of CyCAST proteins (except TnsD) with control crRNA for CyPSP1. |
| 200848 | pMSIP124: pDonor(CyCAST)_0.5kb-cargo | CyCAST donor harboring non-functional cargo. |
| 200849 | pMSIP103: pTarget(CyCAST) | Target plasmid containing CyPSP1 and attachement site for CyCAST. |
| 200851 | pSCT147: pHelper(AjTn6022) | Inducible expression of AjTn6022 proteins. |
| 200852 | pSCT149: pHelper(AjTn6022)_ΔTnsA | Inducible expression of AjTn6022 proteins (except TnsA). |
| 200853 | pSCT150: pHelper(AjTn6022)_ΔTnsB | Inducible expression of AjTn6022 proteins (except TnsB). |
| 200854 | pSCT151: pHelper(AjTn6022)_ΔTnsC | Inducible expression of AjTn6022 proteins (except TnsC). |
| 200855 | pSCT152: pHelper(AjTn6022)_ΔTniQ | Inducible expression of AjTn6022 proteins (except TniQ). |
| 200859 | pSCT385: pHelper(AjTn6022)_QF-separated | Inducible expression of AjTn6022 proteins. |
| 200860 | pSCT400: pHelper(AjTn6022)_QF-separated_TniQ-K348D | Inducible expression of AjTn6022 proteins. |
| 200861 | pSCT401: pHelper(AjTn6022)_QF-separated_TniQ-K361D | Inducible expression of AjTn6022 proteins. |
| 200862 | pSCT112: pTarget(AjTn6022)_U50D50 | Target plasmid containing attachement site for AjTn6022. |
| 200863 | pSCT91: pR6K-Donor(AjTn6022) | AjTn6022 donor harboring KanR cargo. |
| 200864 | pSCT368: pSC101-Donor(AjTn6022) | AjTn6022 donor harboring KanR cargo. |
| 200865 | pSCT162: pTwinStrep-bdSUMO-AjTn6022_TnsF | AjTn6022_TniQ expression construct for protein purification. |
| 200866 | pSCT148: pHelper(ZooTsy) | Inducible expression of ZooTsy proteins. |
| 200867 | pSCT154: pHelper(ZooTsy)_ΔYRec | Inducible expression of ZooTsy (except YRec) proteins. |
| 200868 | pSCT155: pHelper(ZooTsy)_ΔHTH | Inducible expression of ZooTsy (except HTH) proteins. |
| 200869 | pSCT156: pHelper(ZooTsy)_ΔTnsF | Inducible expression of ZooTsy (except TnsF) proteins. |
| 200872 | pSCT315: pHelper(ZooTsy)_TnsF-Y584F | Inducible expression of ZooTsy proteins. |
| 200873 | pSCT375: pDonor(ZooTsy)_excision-reporter | Reporter for ZooTsy excision. |
| 200874 | pSCT221: pR6K-Donor(ZooTsy)_H1:12_E1:135_E2:20_H2:12 | ZooTsy donor harboring KanR cargo. |
| 200875 | pSCT384: pSC101-Donor(ZooTsy) | ZooTsy donor harboring KanR cargo. |
| 200876 | pSCT81: pTarget(ZooTsy) | Target plasmid containing attachement site for ZooTsy. |
| 200877 | pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F | ZooTsy_TnsF expression construct for protein purification. |