Role of the 5' end phosphorylation state for small RNA stability and target RNA regulation in bacteria.
Schilder A, Gorke B
Nucleic Acids Res. 2023 Mar 29:gkad226. doi: 10.1093/nar/gkad226.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200836 | pYG205 | Transcribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivatives |
| 200837 | pYG215 | Transcribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage. |
| 200838 | Z956 | Host strain for plasmids pYG205 and pYG215 or derivatives of the latter |