Uncovering receptor-ligand interactions using a high-avidity CRISPR activation screening platform.
Yang L, Sheets TP, Feng Y, Yu G, Bajgain P, Hsu KS, So D, Seaman S, Lee J, Lin L, Evans CN, Guest MR, Chari R, St Croix B
Sci Adv. 2024 Feb 16;10(7):eadj2445. doi: 10.1126/sciadv.adj2445. Epub 2024 Feb 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200832 | pGMC00028 (aka pTS0057) | AAVS1 guide RNA cloned into the lentiSAM puro backbone |
| 200834 | pGMC00029 (aka pRC0610) | Lentiviral guide RNA expression plasmid for CRISPR activation with puromycin/BFP |
| 207471 | FNLCR CRISPRa Cell Surface | CRISPR-based activation library with guide RNAs targeting all cell surface proteins. |
| 209673 | pGMC00030 (aka pRC0707) | Lentiviral vector expressing PP7-P65-HSF1 |