Fluorescent Biosensor for Measuring Ras Activity in Living Cells.
Weeks R, Zhou X, Yuan TL, Zhang J
J Am Chem Soc. 2022 Sep 28;144(38):17432-17440. doi: 10.1021/jacs.2c05203. Epub 2022 Sep 19.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
205567 | pcDNA3.1(+)-RasAR-NES | Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol. |
205568 | pcDNA3.1(+)-RasAR(R89L)-NES | RasAR negative-control mutant plasmid. Targeted to cytosol. |
205569 | pcDNA3.1(+)-PM-RasAR | Plasma membrane-targeted RasAR. |
205570 | pcDNA3.1(+)-Golgi-RasAR | Golgi membrane-targeted RasAR. |