New PALM-compatible integration vectors for use in the Gram-positive model bacterium Bacillus subtilis
Altinoglu I, Carballido-Lopez R
bioRxiv 2023
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200418 | pIA027 | amyE::(dronPA cm), for dronPA fusions. PALM-compatible integration vector for Bacillus subtilis. Includes dronPA photoswitchable FP with multi cloning sites (MCS) for fast and interchangeable cloning. |
| 200419 | pIA028 | thrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloning |
| 200420 | pIA033 | thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning. |
| 200421 | pIA034 | amyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning. |
| 200424 | pIA030 | thrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis. |
| 200425 | pIA036 | amyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis. |
| 200426 | pIA035 | amyE::(Ppen-lacIΔ11-dronPA cm). PALM integration vector includes dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis. |