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Chemically Modified mocRNAs for Highly Efficient Protein Expression in Mammalian Cells.

Aditham A, Shi H, Guo J, Zeng H, Zhou Y, Wade SD, Huang J, Liu J, Wang X
ACS Chem Biol. 2022 Dec 16;17(12):3352-3366. doi: 10.1021/acschembio.1c00569. Epub 2022 Jan 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
200393pCS2_hMGFP-60AA plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.
200394pCS2_RFP-60APlasmid IVT template encoding RFP. NOTE: In the manuscript, "mCherry" was listed as the internal control for multiple fluorescence-based experiments, but it was actually RFP.
200395pCS2_Firefly_luc-60AA plasmid IVT template containing a Firefly luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.
200396pCS2_Firefly_degron-60AA plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.
200397pCS2_Renilla_luc-60AA plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Antibodies from Article