Engineering oncogenic hotspot mutations on SF3B1 via CRISPR-directed PRECIS mutagenesis.
Fernandez M, Yu L, Jia Q, Wang X, Hart KL, Jia Z, Lin RJ, Wang L
Cancer Res Commun. 2024 Aug 28. doi: 10.1158/2767-9764.CRC-24-0145.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200317 | pCMV-PE5max-SF3B1-K700E | Expresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection. |
| 200318 | pLenti-SF3B1-K700E-reporter | A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette. |