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An evolved AAV variant enables efficient genetic engineering of murine T cells.

Nyberg WA, Ark J, To A, Clouden S, Reeder G, Muldoon JJ, Chung JY, Xie WH, Allain V, Steinhart Z, Chang C, Talbot A, Kim S, Rosales A, Havlik LP, Pimentel H, Asokan A, Eyquem J
Cell. 2023 Jan 19;186(2):446-460.e19. doi: 10.1016/j.cell.2022.12.022. Epub 2023 Jan 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
200257pArk313Ark313 rep/cap
200261pAAV-U6/sgClta-sfGFP-CltaU6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

Antibodies from Article