Autocatalytic base editing for RNA-responsive translational control.

Gayet RV, Ilia K, Razavi S, Tippens ND, Lalwani MA, Zhang K, Chen JX, Chen JC, Vargas-Asencio J, Collins JJ
Nat Commun. 2023 Mar 11;14(1):1339. doi: 10.1038/s41467-023-36851-z. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
200411	pDV24_AAV2_DART-VADAR_iRFP720-sensor	AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA
200410	pDV06.2c_DART-VADAR_iRFP720-sensor	pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2
200409	pDV05.2_pEF1a-iRFP720_secreted	Expresses a secreted version of iRFP720 constitutively in mammalian cells
200408	pDV05.1_pEF1a-iRFP720_frameshifted	Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein
200407	pDV05.0_pEF1a-iRFP720	Expresses iRFP720 constitutively in mammalian cells
200406	pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES	Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells
200405	pDV10_pCMV-ADAR1-p150	Expresses ADAR1 (p150 isoform) constitutively in mammalian cells
200404	pDV09_pCMV-ADAR1-p110	Expresses ADAR1 (p110 isoform) constitutively in mammalian cells
200403	pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES	Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection
200402	pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES	Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection