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Mapping Interactions of the Intrinsically Disordered C-Terminal Regions of Tetrameric p53 by Segmental Isotope Labeling and NMR.

Krois AS, Park S, Martinez-Yamout MA, Dyson HJ, Wright PE
Biochemistry. 2022 Dec 6;61(23):2709-2719. doi: 10.1021/acs.biochem.2c00528. Epub 2022 Nov 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
200311H6GB1tevP53SS(1-393)Bacterial expression of human superstable p53 as His6GB1tev fusion
200312H6GB1tevP53(1-303)IntNBacterial expression of P53 NTAD-DBD for segmental labeling
200313H6GB1tevP53(304-393)bacterial expression of p53 TET-CTD
200314H6GB1tevP53(363-393)bacterial expression of P53 CTD
200315H6GB1tev-IntC-P53(304-393)bacterial expression of P53 TETCTD for segmental labeling
200316H6SUMO-IntC-P53(304-393)bacterial expression of P53 TET-CTD for segmental labeling

Antibodies from Article