Mapping Interactions of the Intrinsically Disordered C-Terminal Regions of Tetrameric p53 by Segmental Isotope Labeling and NMR.
Krois AS, Park S, Martinez-Yamout MA, Dyson HJ, Wright PE
Biochemistry. 2022 Dec 6;61(23):2709-2719. doi: 10.1021/acs.biochem.2c00528. Epub 2022 Nov 15.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
200311 | H6GB1tevP53SS(1-393) | Bacterial expression of human superstable p53 as His6GB1tev fusion |
200312 | H6GB1tevP53(1-303)IntN | Bacterial expression of P53 NTAD-DBD for segmental labeling |
200313 | H6GB1tevP53(304-393) | bacterial expression of p53 TET-CTD |
200314 | H6GB1tevP53(363-393) | bacterial expression of P53 CTD |
200315 | H6GB1tev-IntC-P53(304-393) | bacterial expression of P53 TETCTD for segmental labeling |
200316 | H6SUMO-IntC-P53(304-393) | bacterial expression of P53 TET-CTD for segmental labeling |