Structure reveals why genome folding is necessary for site-specific integration of foreign DNA into CRISPR arrays.
Santiago-Frangos A, Henriques WS, Wiegand T, Gauvin CC, Buyukyoruk M, Graham AB, Wilkinson RA, Triem L, Neselu K, Eng ET, Lander GC, Wiedenheft B
Nat Struct Mol Biol. 2023 Nov;30(11):1675-1685. doi: 10.1038/s41594-023-01097-2. Epub 2023 Sep 14.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192207 | pWT-IFl_WT-IFr | Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays. |
| 192208 | pScr-IFl_WT-IFr | Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays. |
| 192209 | pCon-IFl_Con-IFr | Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays. |
| 192210 | pCon-IFl_Con-IEr | Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays. |
| 192211 | pCon-IFl_Con-ICr | Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays. |
| 192212 | pCon-IFl_Con-IIAr | Consensus I-F CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays. |
| 192213 | pCon-IEl_Con-IEr | Consensus I-E CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays. |
| 192214 | pCon-ICl_Con-ICr | Consensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays. |
| 192215 | pCon-IIAl_Con-IIAr | Consensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays. |
| 192216 | pX1-IFl_Con-IFr | Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays. |
| 192217 | pX2-IFl_Con-IFr | Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays. |
| 200183 | WT_IFL_G1A-T2A_R | WT PA14 I-F Leader and G1A-T2A Repeat |
| 200184 | WT_IFL_A28C_R | WT PA14 I-F Leader and A28C Repeat |
| 200185 | WT_IFL_G1A-T2A-A28C_R | WT PA14 I-F Leader and G1A-T2A-A28C Repeat |
| 200213 | pRAW-457 | Strep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A) |
| 200214 | pRAW-458 | Strep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A) |
| 200215 | pRAW-459 | Strep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A) |
| 200216 | pRAW-460 | Strep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D) |
| 200217 | pRAW-461 | Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E) |
| 200218 | pRAW-462 | Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D) |