Structure reveals why genome folding is necessary for site-specific integration of foreign DNA into CRISPR arrays.
Santiago-Frangos A, Henriques WS, Wiegand T, Gauvin CC, Buyukyoruk M, Graham AB, Wilkinson RA, Triem L, Neselu K, Eng ET, Lander GC, Wiedenheft B
Nat Struct Mol Biol. 2023 Nov;30(11):1675-1685. doi: 10.1038/s41594-023-01097-2. Epub 2023 Sep 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200183 | WT_IFL_G1A-T2A_R | WT PA14 I-F Leader and G1A-T2A Repeat |
| 200184 | WT_IFL_A28C_R | WT PA14 I-F Leader and A28C Repeat |
| 200185 | WT_IFL_G1A-T2A-A28C_R | WT PA14 I-F Leader and G1A-T2A-A28C Repeat |
| 200213 | pRAW-457 | Strep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A) |
| 200214 | pRAW-458 | Strep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A) |
| 200215 | pRAW-459 | Strep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A) |
| 200216 | pRAW-460 | Strep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D) |
| 200217 | pRAW-461 | Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E) |
| 200218 | pRAW-462 | Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D) |