Engineering variants of the I-SceI homing endonuclease with strand-specific and site-specific DNA-nicking activity.
Niu Y, Tenney K, Li H, Gimble FS
J Mol Biol. 2008 Sep 26;382(1):188-202. doi: 10.1016/j.jmb.2008.07.010. Epub 2008 Jul 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195613 | pET15b I-SceI (Ctermhis -MH) | Expresses the wild-type I-SceI homing endonuclease from the T7 promoter |
| 195614 | pET15b I-SceI (Ctermhis) K122I | Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity |
| 195615 | pET15b I-SceI (Ctermhis) K223I | Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity |