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Identification of Lys-403 in the PI-SceI homing endonuclease as part of a symmetric catalytic center.

Gimble FS, Duan X, Hu D, Quiocho FA
J Biol Chem. 1998 Nov 13;273(46):30524-9. doi: 10.1074/jbc.273.46.30524. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
195612pET PI-SceI C-His K403AExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutation

Antibodies from Article