Amino acid residues in both the protein splicing and endonuclease domains of the PI-SceI intein mediate DNA binding.
He Z, Crist M, Yen H, Duan X, Quiocho FA, Gimble FS
J Biol Chem. 1998 Feb 20;273(8):4607-15. doi: 10.1074/jbc.273.8.4607.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195610 | pET PI-SceI C-His | Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tag |
| 195611 | pET PI-SceI C-His K301A | Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutation |