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Substitutions in conserved dodecapeptide motifs that uncouple the DNA binding and DNA cleavage activities of PI-SceI endonuclease.

Gimble FS, Stephens BW
J Biol Chem. 1995 Mar 17;270(11):5849-56. doi: 10.1074/jbc.270.11.5849. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
195607pET23PI-Sce ESARCExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter
195608pET23PI-Sce ESARC D218AExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutation
195609pT7PI-Sce ESARC D326AExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutation

Antibodies from Article