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Rapid generation of C. elegans single-copy transgenes combining RMCE and drug selection.

Nonet ML
Genetics. 2023 Apr 20:iyad072. doi: 10.1093/genetics/iyad072. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
200066pHygG2hygR selection nls-mNG marked rapid RMCE integration vector
200069pHygG2rhygR selection nls-mNG marked rapid RMCE integration vector
200071pHygR2hygR selection nls-mScarlet marked rapid RMCE integration vector
200072pHygR2rhygR selection nls-mScarlet marked rapid RMCE integration vector
200073pHygCG2hygR selection mNG marked rapid RMCE integration vector
200074pHygCG2rhygR selection mNG marked rapid RMCE integration vector
200075pHygRR2hygR selection mScarlet 2X marked rapid RMCE integration vector
200076pHygRR2rhygR selection mScarlet 2X marked rapid RMCE integration vector
200077pHygO2hygR selection unmarked rapid RMCE integration vector
200079pHygO2rhygR selection unmarked rapid RMCE integration vector
200219pHygG6hygR selection GFP marked rapid RMCE integration vector
200220pHygG8hygR selection nls-mNG marked rapid RMCE integration vector without loxP site
200221pHygR8hygR selection nls-mScarlet marked rapid RMCE integration vector without loxP site
200222pNeoG2NeoR nls-mNG marked rapid RMCE integration vector
200223pNeoR2NeoR nls-scarlet marked rapid RMCE integration vector
200224pPuroG1puroR nls-mNG marked rapid RMCE integration vector

Antibodies from Article