Stable, fluorescent markers for tracking synthetic communities and assembly dynamics.

Jorrin B, Haskett TL, Knights HE, Martyn A, Underwood TJ, Dolliver J, Ledermann R, Poole PS
Microbiome. 2024 May 7;12(1):81. doi: 10.1186/s40168-024-01792-2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
222336	pFlp-Tc	flp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3
222335	pFlp-Gm	flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3
222334	pFlp-Km	flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3
201019	pTn7-SCOUT22.YB	pLv1-pUC18R6KT-miniTn7-Km-sYFP2-TagBFP, KmR, ApR
201018	pTn7-SCOUT22.RY	pLv1-pUC18R6KT-miniTn7-Km-mCherry-sYFP2, KmR, ApR
201015	pTn7-SCOUT14	pLv1-pUC18R6KT-miniTn7-aad9, SpR, StR, ApR
201009	pTn7-SCOUT14.Y	pLv1-pUC18R6KT-miniTn7-aad9-sYFP2, SpR,StR, ApR
201008	pTn7-SCOUT21.YB	pLv1-pUC18R6KT-miniTn7-Gm-sYFP2-TagBFP, GmR, ApR
201007	pTn7-SCOUT21.RB	pLv1-pUC18R6KT-miniTn7-Gm-mCherry-TagBFP, GmR, ApR
201006	pTn7-SCOUT21.RY	pLv1-pUC18R6KT-miniTn7-Gm-mCherry-sYFP2, GmR, ApR