A Split-Intein-Based Method for the Efficient Production of Circularized Nanodiscs for Structural Studies of Membrane Proteins.
Miehling J, Goricanec D, Hagn F
Chembiochem. 2018 Sep 17;19(18):1927-1933. doi: 10.1002/cbic.201800345. Epub 2018 Jul 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199766 | pET28a-cMSPdH4-6 | Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag) |
| 199767 | pET28a-cMSPdH45 | Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag) |
| 199768 | pET28a-cMSP1D1 | Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag) |
| 199769 | pET28a-cMSP26 | Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag) |
| 199771 | pET28a-cMSPdH5 | Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag) |