Quantification of elongation stalls and impact on gene expression in yeast.
Hou W, Harjono V, Harvey AT, Subramaniam AR, Zid BM
bioRxiv. 2023 Mar 20:2023.03.19.533377. doi: 10.1101/2023.03.19.533377. Preprint.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199755 | pAG306-pTet07-nLuc | Control construct of elongation reporter to quantify elongation of YFP |
| 199756 | pAG306-pTet07-synYFP[TTG/AGA]-nLuc | Elongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stalling |
| 199757 | pAG306-pTet07-leuYFP[CTT]-nLuc | Elongation reporter construct to quantify elongation of synYFP[CTT] |
| 199758 | pAG306-pTet07-leuYFP[CTC]-nLuc | Elongation reporter construct to quantify elongation of synYFP[CTC] |
| 199759 | pAG306-pTet07-synYFP[TTG/AGA]-4xArg[CGA]-nLuc | Elongation reporter construct to quantify elongation duration of 4_CGA stalling |
| 199760 | pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc | Elongation reporter construct to quantify elongation duration of 6_CGA stalling |
| 199761 | pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc | Elongation reporter construct to quantify elongation duration of 6_AGA stalling |