Structure-guided AAV capsid evolution strategies for enhanced CNS gene delivery.
Gonzalez TJ, Mitchell-Dick A, Blondel LO, Fanous MM, Hull JA, Oh DK, Moller-Tank S, Castellanos Rivera RM, Piedrahita JA, Asokan A
Nat Protoc. 2023 Sep 21. doi: 10.1038/s41596-023-00875-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199743 | pMCV | Minimal cloning vector |
| 199744 | pITR-AAV-Rep2Cap9-GFP | Expresses AAV2 Rep and AAV9 Cap, with GFP replacing a region of Cap, within AAV2 ITRs. |
| 199745 | pAAV.cc81-mut | Expresses AAV2 Rep and AAV.cc81 Cap |
| 199746 | pAAV.cc84-mut | Expresses AAV2 Rep and AAV.cc84 Cap |
| 200182 | pAAV9-null | Expresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 site |