New T-DNA binary vectors with NptII selection and RUBY reporter for efficient maize transformation and targeted mutagenesis.
Lee K, Kang M, Ji Q, Grosic S, Wang K
Plant Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199721 | pKL2351 | A pVS1-based T-DNA binary vector and destination vector for Gateway cloning. |
| 199722 | pCBL101 | A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. |
| 199723 | pCBL101-RUBY | Expresses betalain biosynthesis genes under CaMV 35S promoter |
| 199724 | pCBL101.1 | A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed. |
| 199725 | pCBL101gw | A pVS1-based T-DNA binary vector and destination vector for Gateway cloning with NPTII as a plant selectable marker gene. |
| 199726 | pCBLgRNA | Gateway entry vector for single gRNA under OsU3 promoter. |
| 199727 | pCBLsgRNA1 | Golden Gate entry vector; 1st gRNA under OsU3 promoter |
| 199728 | pCBLsgRNA2 | Golden Gate entry vector; 2nd gRNA under OsU3 promoter |
| 199729 | pCBLsgRNA3 | Golden Gate entry vector; 3rd gRNA under TaU3 promoter |
| 199730 | pCBLsgRNA4 | Golden Gate entry vector; 4th gRNA under ZmU6-C1 promoter |
| 199731 | pCBLsgRNA5 | Golden Gate entry vector; 5th gRNA under ZmU6-C3 promoter |
| 199732 | pCBLsgRNA6 | Golden Gate entry vector; 6th gRNA under OsU6.1 promoter |
| 199733 | pCBLsgRNA7 | Golden Gate entry vector; 7th gRNA under OsU6.2 promoter |
| 199734 | pCBLsgRNA8 | Golden Gate entry vector; 8th gRNA under TaU6 promoter |
| 199735 | pCBL101gg | Golden Gate recipient vector for transgene overexpression |
| 199736 | pCBLggPro | Golden Gate entry vector for promoter cloning |
| 199737 | pCBLggCDS | Golden Gate entry vector for gene of interest cloning |
| 199738 | pCBLggTerm | Golden Gate entry vector for terminator cloning |
| 210379 | pCBL102-RUBY | Expresses betalain biosynthesis genes under CaMV 35S promoter |