Heat shock transcription factors demonstrate a distinct mode of interaction with mitotic chromosomes.
Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS
Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200192 | Halo-HSF2 | Expression of full length HSF2 |
| 200193 | H2DBD-H1-Halo | Expression of HSF1 with HSF2 DBD |
| 200194 | Halo-Sox13 | Expression of full length Sox13 |
| 200195 | Halo-S2S13DBD | Expression of Sox2 with Sox13 DBD |
| 200196 | Halo-S13S2DBD | Expression of Sox13 with Sox2 DBD |
| 200197 | NLS-Halo | Expression for SV40 NLS |
| 200198 | NLS-Halo-HSF2 | Expression of full length HSF2 with SV40 NLS |
| 200199 | Halo-H2DBD-HR | Expression of truncated HSF2 (DBD and HR) |
| 200200 | Halo-H2DBD | Expression of truncated HSF2 (DBD) |
| 200201 | NES-Halo | Expression of 3X HIV NES |
| 200202 | HA-Halo-HSF2 | Expression of full length HSF2 |
| 200203 | H1DBD-Halo-HA | Expression of truncated HSF1 (DBD and HR) |
| 200204 | H1DBD-HR-Halo | Expression of truncated HSF1 (DBD and HR) |
| 200205 | Halo-H1DBD-H2 | Expression of HSF2 with HSF1 DBD |
| 200206 | HA-Halo-H2DBD | Expression of truncated HSF2 (DBD) |
| 200207 | HSF1 KO | gRNA for HSF1 KO |
| 200208 | HSF2 KO | gRNA for HSF2 KO |