Lysosomal GPCR-like protein LYCHOS signals cholesterol sufficiency to mTORC1.
Shin HR, Citron YR, Wang L, Tribouillard L, Goul CS, Stipp R, Sugasawa Y, Jain A, Samson N, Lim CY, Davis OB, Castaneda-Carpio D, Qian M, Nomura DK, Perera RM, Park E, Covey DF, Laplante M, Evers AS, Zoncu R
Science. 2022 Sep 16;377(6612):1290-1298. doi: 10.1126/science.abg6621. Epub 2022 Aug 25.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199633 | pLVX LYCHOS (GPR155) WT-FLAG | Lentiviral construct to stably express LYCHOS (GPR155) WT |
| 199660 | pRK5-LYCHOS (GPR155) WT-HA | Transient expression of LYCHOS (GPR155) WT |
| 199661 | pRK5-LYCHOS (GPR155) FP>IA-HA | Transient expression of LYCHOS (GPR155) FP>IA mutant |
| 199662 | pRK5-LYCHOS (GPR155) E48Q-HA | Transient expression of LYCHOS (GPR155) E48Q mutant |
| 199674 | pRK5-LYCHOS (GPR155) F43I-HA | Transient expression of LYCHOS (GPR155) F43I mutant |
| 199675 | pRK5-LYCHOS (GPR155) P44A-HA | Transient expression of LYCHOS (GPR155) P44A mutant |
| 199676 | pRK5 LYCHOS (GPR155) Y551A-HA | Transient expression of LYCHOS (GPR155) Y551A mutant |
| 199677 | pRK5-LYCHOS (GPR155) 4CA-HA | Transient expression of LYCHOS (GPR155) C595.604.629.638A mutant |
| 199678 | pET28a LZ control-FLAG | Bacterial expression of Leucine Zipper (LZ) control-Flag |
| 199679 | pET28a LZ LED WT-FLAG | Bacterial expression of Leucine Zipper (LZ) LYCHOS LED-Flag |
| 199680 | pET28a LZ LED Y551A-FLAG | Bacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag |
| 199681 | pET28a LZ LED 4CA-FLAG | Bacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-Flag |
| 199682 | pRK5 LYCHOS ∆LED-HA | Transient expression of LYCHOS (GPR155) ∆LED-HA |
| 199683 | pRK5-LYCHOS (GPR155) ∆DEP-HA | Transient expression of LYCHOS (GPR155) ∆DEP-HA |
| 199684 | pRK5-LYCHOS (GPR155) WT-myc | Transient expression of LYCHOS (GPR155) WT |
| 199685 | pRK5-myc Metap2 | Transient expression of Metap2 |
| 199686 | pRK5 LYCHOS (GPR155) ∆Permease-HA | Transient expression of LYCHOS (GPR155) ∆Permease |
| 199687 | pFB-CG-LYCHOS WT-HA-GFP | Expression of LYCHOS WT in insect cells |
| 199688 | pFB-CG-LYCHOS FP>IA-HA-GFP | Expression of LYCHOS FP>IA mutant in insect cells |
| 199747 | pLVX LYCHOS-FLAG-TiD | Lentiviral construct to stably express LYCHOS (GPR155) TurboID |
| 199748 | pLVX NPC1-FLAG-TiD | Lentiviral construct to stably express NPC1 TurboID |
| 199749 | pLVX FLAG-SLC38A9.1 | Lentiviral construct to stably express SLC38A91.1 |
| 199750 | pLJM1-Lamp1 FLAG | Lentiviral construct to stably express Lamp1 |
| 209314 | pLKO shLYCHOS/FLAG Metap2 | Lentiviral construct expressing FLAG Metap2 and shRNA against LYCHOS |
| 209315 | pLKO shLYCHOS/LYCHOS WT-FLAG | Lentiviral construct expressing shRNA against LYCHOS and LYCHOS WT-FLAG (resistant to shRNA) |
| 209316 | pLKO shLuc/FLAG Metap2 | Lentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA) |