Development of a CRISPR-Cas12a system for efficient genome engineering in clostridia.
Zhang Y, Kubiak AM, Bailey TS, Claessen L, Hittmeyer P, Dubois L, Theys J, Lambin P
Microbiol Spectr. 2023 Nov 10:e0245923. doi: 10.1128/spectrum.02459-23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199563 | pPIPL12-FnCas12a | Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia |
| 199564 | pPfetFn-Target_v2 | gRNA expression for FnCas12a in E. coli and clostridia |