Engineered allostery in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells.
Lee SY, Cheah JS, Zhao B, Xu C, Roh H, Kim CK, Cho KF, Udeshi ND, Carr SA, Ting AY
Nat Methods. 2023 May 15. doi: 10.1038/s41592-023-01880-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199545 | V5-LOV-Turbo-NES | expresses LOV-Turbo in the mammalian cytosol, lentiviral vector |
| 199663 | V5-LOV-Turbo-NLS | expresses LOV-Turbo in the mammalian nucleus, lentiviral vector |
| 199664 | V5-LOV-Turbo-ERM | expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector |
| 199665 | OMM-V5-LOV-Turbo | expresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vector |
| 199666 | Mito-LOV-Turbo-V5 | expresses LOV-Turbo in the mammalian mitochondria, lentiviral vector |
| 199667 | LOV-Turbo-V5_pRS415 | expresses LOV-Turbo in the yeast cytosol |
| 199668 | LOV-Turbo-His6_pET21a | expresses LOV-Turbo in bacteria |
| 199669 | AAV_pSyn_Mito-LOV-Turbo-V5 | expresses Mito-LOV-Turbo-V5 in neurons, AAV vector |
| 199670 | AAV_CAG_V5-LOV-Turbo-NES | expresses V5-LOV-Turbo-NES in mammalian cells, AAV vector |
| 199671 | Myc-βarrestin2-LOV-Turbo_pCDNA3 | expresses Myc-βarrestin2-LOV-Turbo in mammalian cells |
| 199672 | AAV_CMV_CCR6-Nanoluc-V5 | expresses CCR6-NanoLuc-V5 in mammalian cells, AAV vector |
| 199673 | Aga2p-LOV-Turbo1-myc_pCTCON2 | expresses pre-evolved version of LOV-Turbo on the yeast surface |